PRIMARY STRUCTURE-ANALYSIS OF A DUPLICATED REGION IN THE AMPLIFIABLE AUD6 LOCUS OF STREPTOMYCES-AMBOFACIENS DSM40697

In Streptomyces ambofaciens, an amplifiable unit of DNA (AUD6) contain s two homologous sequences, one located on the right extremity of the AUD (SIR), the other being internal (IHS). This paper presents the mol ecular analysis of this duplication. The nucleotide sequences are almo st identical (95%) and each contains an ORF of about 330 codons, the t wo ORFs being nearly identical. The two hypothetical proteins, deduced from these sequences, show about 30% identity with different bacteria l repressors. They also show a particularly strong similarity (90% ide ntity between the full-length sequences) with hypothetical proteins of Streptomyces lividans 66 encoded by sequences also present on an ampl ifiable DNA region (AUD1).