KINETIC-PROPERTIES OF YEAST LYSINE PERMEASES CODED BY GENES ON MULTI-COPY VECTORS

Authors
SYCHROVA H MATEJCKOVA A KOTYK A
Citation
H. Sychrova et al., KINETIC-PROPERTIES OF YEAST LYSINE PERMEASES CODED BY GENES ON MULTI-COPY VECTORS, FEMS microbiology letters, 113(1), 1993, pp. 57-61
Citations number
11
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
113
Issue
1
Year of publication
1993
Pages
57 - 61
Database
ISI
SICI code
0378-1097(1993)113:1<57:KOYLPC>2.0.ZU;2-K
Abstract
Amplification of the LYP1 transport system in the plasma membrane of S accharomyces cerevisiae did not change the substrate specificity, the affinity and the pH optimum of the transport system for lysine, but si gnificantly increased the uptake velocity and accumulation ratio. The dependence of active lysine uptake and accumulation on pH is probably given by the properties of the permease itself rather than by the valu e of available protonmotive force.