Jj. Gagliardino et al., IMMUNOCYTOCHEMICAL DETECTION OF BROMODEOXYURIDINE IN TISSUES OF THE TOAD BUFO-ARENARUM HENSEL, Biotechnic & histochemistry, 68(5), 1993, pp. 302-304
We present a modified protocol for the immunocytochemical identificati
on of 5-bromo-2'-deoxyuridine (BrdU) as an indicator of cell replicati
on in different tissues of the toad, Bufo arenarum Hensel. Animals wer
e sacrificed 60 min after BrdU (5 mg/100 g body weight) was injected i
nto the dorsal lymph sac. The tissues were fixed in Carnoy's fluid and
stained by the immunoperoxidase method using an anti-BrdU monoclonal
antibody. This protocol can be used safely for the study of cell repli
cation in toads and other Anura amphibia.