IMMUNOCYTOCHEMICAL DETECTION OF BROMODEOXYURIDINE IN TISSUES OF THE TOAD BUFO-ARENARUM HENSEL

Authors
GAGLIARDINO JJ DUMM CLAG BIANCHI CE LUNA GC
Citation
Jj. Gagliardino et al., IMMUNOCYTOCHEMICAL DETECTION OF BROMODEOXYURIDINE IN TISSUES OF THE TOAD BUFO-ARENARUM HENSEL, Biotechnic & histochemistry, 68(5), 1993, pp. 302-304
Citations number
5
Categorie Soggetti
Cytology & Histology","Biothechnology & Applied Migrobiology
Journal title
Biotechnic & histochemistry → ACNP
ISSN journal
10520295
Volume
68
Issue
5
Year of publication
1993
Pages
302 - 304
Database
ISI
SICI code
1052-0295(1993)68:5<302:IDOBIT>2.0.ZU;2-E
Abstract
We present a modified protocol for the immunocytochemical identificati on of 5-bromo-2'-deoxyuridine (BrdU) as an indicator of cell replicati on in different tissues of the toad, Bufo arenarum Hensel. Animals wer e sacrificed 60 min after BrdU (5 mg/100 g body weight) was injected i nto the dorsal lymph sac. The tissues were fixed in Carnoy's fluid and stained by the immunoperoxidase method using an anti-BrdU monoclonal antibody. This protocol can be used safely for the study of cell repli cation in toads and other Anura amphibia.