ORIENTATION OF SMALL SPECIMENS FOR CRYOSECTIONING

A simple technique is introduced to achieve symmetrically Oriented fro zen sections of small specimens such as young fish or frog larvae. Sma ll samples are especially difficult to orient if they are already froz en to the chuck in a freezing microtome. Orientation of the sample in a mold filled with embedding medium prior to freezing permits sectioni ng as well as easy labeling and storage of the specimens. The use of a stereo microscope during orientation is optional.