AUTORADIOGRAPHIC LOCALIZATION OF STRYCHNINE-SENSITIVE GLYCINE RECEPTOR IN BOVINE ADRENAL-MEDULLA

The presence of an uptake system and a functional glycine receptor in adrenal medulla chromaffin cells was investigated using an autoradiogr aphic technique in adrenal gland slices. Specific [H-3]glycine binding was observed in both adrenal cortex and medulla slices, while only sp ecific binding of [H-3]strychnine was seen only in chromaffin cells an d was not associated with cortical cells. [H-3]Glycine binding sites i n the cortex are apparently different from those of [H-3]strychnine bi nding sites in the medulla since excess strychnine does not displace [ H-3]glycine from adrenal cortex but does so from medulla. This differe nce supports biochemical evidence for glycine transport into medulla c ells and glycine receptor sites on the chromaffin cell membrane.