FACILE PREPARATION OF RAT INTESTINAL-MUCOSA FOR ASSAY OF MUCOSAL ENZYME-ACTIVITY

Intestinal mucosa of rats was prepared by squeezing the frozen and tha wed intestine. The method was much easier compared to the conventional method in which intestine was cut longitudinally or everted and mucos a was scraped. Jejunal mucosal weight prepared by the two methods was not different, but ileal mucosal weight prepared by squeezing was sign ificantly heavier than that by scraped. No significant difference was observed between activity of some brush border enzymes (arylamidase, a minopeptidase, alkaline phosphatase, and sucrase) in mucosa prepared b y squeezing and that by conventional scraping method in jejunum. Activ ity of brush border aminopeptidase and sucrase in ileal mucosa prepare d by squeezing was significantly higher than that prepared by scraping .