MITOGENIC EFFECT OF LYMPH, AMNIOTIC AND ALLANTOIC FLUID IN BOVINE FETAL MYOBLASTS

Amniotic and allantoic fluids, as well as afferent lymph (tissue fluid ), stimulated DNA synthesis in bovine fetal myoblasts (BFM) at low con centrations (5 %). Higher doses abolished the mitostimulatory effect o f these fluids and led toward inhibition of [H-3]mTdR incorporation in to DNA. These effects were much more evident (particularly in the case of amniotic fluid) when fluids were lyophylized prior to the experime nt. BFM protein synthesis was accelerated by lymph in a dose dependent manner.