GENOTYPING OF OVINE BETA-LACTOGLOBULIN ALLELES A AND B USING THE POLYMERASE CHAIN-REACTION

Authors
SCHLEE P KRAUSE I ROTTMANN O
Citation
P. Schlee et al., GENOTYPING OF OVINE BETA-LACTOGLOBULIN ALLELES A AND B USING THE POLYMERASE CHAIN-REACTION, Archiv fur Tierzucht, 36(5), 1993, pp. 519-523
Citations number
NO
Categorie Soggetti
Agriculture Dairy & AnumalScience
Journal title
Archiv fur Tierzucht → ACNP
ISSN journal
00039438
Volume
36
Issue
5
Year of publication
1993
Pages
519 - 523
Database
ISI
SICI code
0003-9438(1993)36:5<519:GOOBAA>2.0.ZU;2-#
Abstract
A method for the determination of beta-lactoglobulin alleles A and B i n sheep is presented. It is based on the polymerase chain reaction (PC R) and a RsaI polymorphism located in the codon of amino acid 20. DNA was extracted from white blood cells and hair roots. The method allows genotyping of male and non lactating animals. The gene frequency of b eta-lactoglobulin alleles A and B was 0.76 and 0.24 in 38 Bavarian Mil k Sheep.