THE EFFECT ON RECOMBINANT PROTEIN-PRODUCTION OF REINSTATING NATIVE INTRONS IN THE CODING SEQUENCE OF HUMAN FACTOR-VIII

Authors
WEBB E NISBET I
Citation
E. Webb et I. Nisbet, THE EFFECT ON RECOMBINANT PROTEIN-PRODUCTION OF REINSTATING NATIVE INTRONS IN THE CODING SEQUENCE OF HUMAN FACTOR-VIII, Biotechnology letters, 15(9), 1993, pp. 889-894
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
15
Issue
9
Year of publication
1993
Pages
889 - 894
Database
ISI
SICI code
0141-5492(1993)15:9<889:TEORPO>2.0.ZU;2-0
Abstract
Reinstating two naturally occurring intervening sequences of the human factor VIII gene into a B domain-deleted factor VIII cDNA sequence, e ither as separate entities or in combination, revealed slightly elevat ed protein production in the case of intron 8, while the addition of i ntron 16 markedly reduced protein levels. This reduction may be due to decreased levels of mRNA.