RANDOM INSERTION OF TN4560 IN STREPTOMYCES-LIVIDANS AND STREPTOMYCES-AVERMITILIS

Authors
IRNICH S CULLUM J
Citation
S. Irnich et J. Cullum, RANDOM INSERTION OF TN4560 IN STREPTOMYCES-LIVIDANS AND STREPTOMYCES-AVERMITILIS, Biotechnology letters, 15(9), 1993, pp. 895-900
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
15
Issue
9
Year of publication
1993
Pages
895 - 900
Database
ISI
SICI code
0141-5492(1993)15:9<895:RIOTIS>2.0.ZU;2-H
Abstract
Pulsed-field gel electrophoresis (PFGE) was used to show that insertio ns of transposon Tn4560 in Streptomyces lividans 66 and the avermectin -producer S. avermitilis are randomly distributed. DNA from the latter strain suffered. degradation during electrophoresis that could be avo ided by modification of the buffer. Inverse PCR primers were developed for Tn4560.