SIMULTANEOUS EXPRESSION OF GENES ENCODING ENDOGLUCANASE AND BETA-GLUCOSIDASE IN ZYMOMONAS-MOBILIS

As a step towards constructing strains of Z. mobilis capable of conver ting cellulose to ethanol, DNA fragments encoding endoglucanase (from Xanthomonas albilineans) and beta-glucosidase (from either X.albilinea ns or Pseudomonas sp.) were linked on the same vector and transferred to Z. mobilis. All clones expressed endoglucanase. Beta-glucosidase wa s only produced by clones containing the Xanthomonas gene, and when tw o copies of this gene were present the beta-glucosidase activity was h igher.