A SIMPLE ASSAY SYSTEM INCORPORATING MONOCLONAL-ANTIBODIES FOR THE DETECTION OF POTATO VIRUS-T

Authors
VERNONSHIRLEY ML BURNS R GEORGE EL HOADLEY ME
Citation
Ml. Vernonshirley et al., A SIMPLE ASSAY SYSTEM INCORPORATING MONOCLONAL-ANTIBODIES FOR THE DETECTION OF POTATO VIRUS-T, Potato research, 36(2), 1993, pp. 83-88
Citations number
10
Categorie Soggetti
Agriculture
Journal title
Potato research → ACNP
ISSN journal
00143065
Volume
36
Issue
2
Year of publication
1993
Pages
83 - 88
Database
ISI
SICI code
0014-3065(1993)36:2<83:ASASIM>2.0.ZU;2-Y
Abstract
Two monoclonal antibodies (McAbs) which react specifically with potato virus T (PVT) were produced and tested for cross reactivity with pota to viruses (A, S, X, Y(o), Y(N), leaf roll), apple stem grooving virus (ASGV) and against healthy sap. They were found to be highly specific for PVT. These two McAbs form the basis of an enzyme-linked immunosor bent assay (ELISA) for the detection of PVT in infected plant material which is of key significance for potato quarantine.