ISOLATION AND CHARACTERIZATION OF 3 CDNAS CODING FOR RAB PROTEINS FROM THE ALBUMIN GLAND OF THE MOLLUSK LYMNAEA-STAGNALIS

Three cDNA clones encoding small GTP-binding proteins, LS-Rab1, LS-Rab 2 and LS-Rab18a were isolated from a cDNA library from the albumen gla nd of the pulmonate snail Lymnaea stagnalis. Comparison of the deduced amino acid sequences with available sequences from the EMBL/Data Data Bank revealed that LS-Rab1 and LS-Rab2 show a sequence identity of 89 -90% to the mammalian Rab1 and Rab2 proteins, and can therefore be reg arded as the L. stagnalis homologs. LS-Rab18a may be considered a new member of the Rab subfamily, closely related to mouse Rab18 (74% amino acid identity). Interestingly, LS-Rab1 and LS-Rab2 share a very high sequence conservation with their mammalian homologs (95-97%) over the first 178-191 N-terminal amino acids, whereas the C-terminal part is a lmost completely divergent, except for their extreme ends (2-4 amino a cids). The implications of these observations for the understanding of Rab-targeting signals are discussed. The LS-rab cDNAs were expressed in COS-7M6 cells. The resulting 22-kDa products were shown to bind GTP . In the albumen gland mRNA, levels of LS-rab1 appeared to be much hig her than those of LS-rab2 and LS-rab18a, suggesting an important role for the LS-Rab1 protein in the albumen gland.