Dm. Dixon et Jr. Traynor, CONVERSION OF DYNORPHIN-A(1-8) TO [LEU5]-ENKEPHALIN IN RAT CENTRAL NERVOUS-TISSUE DURING DEVELOPMENT, Neuropeptides, 25(2), 1993, pp. 121-125
Rat central nervous tissue contains enzymic activity that is able to c
onvert the kappa-receptor preferring opioid peptide dynorphin A(1-8) t
o the delta-mu-receptor preferring opioid peptide [Leu5]enkephalin. Th
e ontogeny of this conversion process has been studied in vitro using
cortex, striatum, cerebellum and spinal cord tissues of the developing
rat brain. Evidence for the enzymic cleavage of the Leu5-Arg6 bond of
dynorphin A(1-8) to afford [Leu5]enkephalin was observed as early as
neonatal day 1. The degree of conversion increased up to day 7, at whi
ch time adult levels were attained. Results in all tissues studied wer
e similar. The relationship between the increase in the conversion pro
cess with age and the ontogeny of opioid peptides and their receptors
may indicate an important role for the process in the developing nervo
us system.