MODELING AND SIMULATION OF THE ENZYMATIC-HYDROLYSIS OF POTATO PULP BYA COMPLEX ENZYME MIXTURE

The enzymatic hydrolysis of potato pulp by a cell-free culture filtrat e of Trichoderma reesei Rut C30 was studied. On the basis of the exper imental data a dynamic unstructured model using Michaelis-Menten kinet ics was developed. This mathematical model describes the enzymatic hyd rolysis in terms of the adsorption and desorption of enzymes on the so lid substrate and with regard to competitive and uncompetitive inhibit ion. The model equations consist of a non-linear system of ordinary di fferential and algebraic equations. Parameter identification was done by dividing the model into submodels and fitting these to experimental data. The simulation results with the model correspond well with the experimental data. Thus the good agreement between simulated and measu red process variables indicates that the model is suitable for descrip tion of the enzymatic hydrolysis. Computations for different operation al conditions show the range of validity and performance of the model. Possibilities for improvements in yield and productivity could be ded uced by model computations.