FUNCTIONAL SIMILARITY BETWEEN ADENOVIRUS E1B 19K GENE AND BCL2 ONCOGENE - MUTANT COMPLEMENTATION AND SUPPRESSION OF CELL-DEATH INDUCED BY DNA-DAMAGING AGENTS

Citation
B. Tarodi et al., FUNCTIONAL SIMILARITY BETWEEN ADENOVIRUS E1B 19K GENE AND BCL2 ONCOGENE - MUTANT COMPLEMENTATION AND SUPPRESSION OF CELL-DEATH INDUCED BY DNA-DAMAGING AGENTS, International journal of oncology, 3(3), 1993, pp. 467-472
Citations number
56
Categorie Soggetti
Oncology
ISSN journal
10196439
Volume
3
Issue
3
Year of publication
1993
Pages
467 - 472
Database
ISI
SICI code
1019-6439(1993)3:3<467:FSBAE1>2.0.ZU;2-T
Abstract
Adenovirus mutants defective in a 19 kD protein (19K gene) encoded by the early region E1B induce DNA fragmentation in a manner similar to t he internucleosomal DNA fragmentation observed during programmed cell death (apoptosis) induced by a number of chemical, physical and biolog ical stimuli. Zn++ ions effectively suppressed DNA fragmentation induc ed by a 19K mutant consistent with their inhibition of DNA fragmentati on during apoptosis. Since the cellular proto-oncogene Bcl2 has been s hown to suppress DNA fragmentation and the resulting programmed cell d eath, we have carried out a functional complementation analysis to det ermine whether the E1B 19 kD protein is functionally similar to the Bc l2 protein. Our results indicate that the DNA fragmentation induced by the 19K mutant can be efficiently suppressed by stable expression of human Bcl2 protein. Further, the 19 kD and Bcl2 proteins also function similarly to suppress DNA fragmentation and cell death induced by the DNA damaging agents cisplatin and UV.