COMPARISON OF SALT STRESS-RESPONSIVE PROTEINS IN ATRIPLEX-AMNICOLA ANTIBODIES AND 2 ECOTYPES OF LEPTOCHLOA-FUSCA (L) KUNTH

Three salt tolerant plant types viz.,Atriplex amnicola, Leptochloa fus ca (L.) Kunth. (Pakistani origin) and Leptochloa fusca (L.) Kunth (Aus tralian origin) were used for a comparative study of altered gene expr ession under salt stress. The plants were aseptically grown in hydropo nic medium and exposed to increasing levels of salt starting from 50mM NaCl to 500mM NaCl. Under these stress treatments there was variable expression of a number of proteins as revealed by SDS-PAGE. Specific a ctivities of plant GDH, GOGAT and GS in response to salt stress were a lso determined. Klebsiella sp.,NIAB-1, a diazotroph isolated from root s of Leptochloa fusca (L.) Kunth was also exposed to 50mM NaCl to 1000 mM NaCl treatments, to examine salt responsive protein profiles. Blots of protein profiles from the three plant types were probed with antib odies raised against p-20 of Atriplex amnicola and p-26 of Klebsiella sp., NIAB- 1, 10 reveal common epitopes among the salt responsive prot eins.