A METHOD TO OBTAIN LARGE QUANTITIES OF TOXOPLASMA-GONDII TACHYZOITES WITH EXTREME PURITY

Authors
DOMZIG W SEGUELA JP BINZ H
Citation
W. Domzig et al., A METHOD TO OBTAIN LARGE QUANTITIES OF TOXOPLASMA-GONDII TACHYZOITES WITH EXTREME PURITY, The Journal of parasitology, 79(4), 1993, pp. 613-615
Citations number
10
Categorie Soggetti
Parasitiology
Journal title
The Journal of parasitology → ACNP
ISSN journal
00223395
Volume
79
Issue
4
Year of publication
1993
Pages
613 - 615
Database
ISI
SICI code
0022-3395(1993)79:4<613:AMTOLQ>2.0.ZU;2-F
Abstract
In order to extract genetic material from the intracellular parasite T oxoplasma gondii, large numbers of organisms free of host cells are ne cessary. A method is described in which the RH strain of T. gondii was cultivated in nonadherent U937 cells. The purification was done by co untercurrent elutriation. The resulting population of 10(10) T. gondii contained only 0.17% host cell material. The method is simple, rapid, and minimizes the use of animals.