EPSTEIN-BARR VIRUS-DERIVED VECTORS FOR TRANSIENT AND STABLE EXPRESSION OF RECOMBINANT PROTEINS

Authors
CACHIANES G HO C WEBER RF WILLIAMS SR GOEDDEL DV LEUNG DW
Citation
G. Cachianes et al., EPSTEIN-BARR VIRUS-DERIVED VECTORS FOR TRANSIENT AND STABLE EXPRESSION OF RECOMBINANT PROTEINS, BioTechniques, 15(2), 1993, pp. 255
Citations number
16
Categorie Soggetti
Biochemical Research Methods
Journal title
BioTechniques → ACNP
ISSN journal
07366205
Volume
15
Issue
2
Year of publication
1993
Database
ISI
SICI code
0736-6205(1993)15:2<255:EVVFTA>2.0.ZU;2-B
Abstract
The transient expression level of foreign proteins in primate cells ca n be enhanced by incorporating the replication elements derived from E pstein-Barr virus (EBV). Specifically, we have constructed expression plasmids with the replication origin region (OriP) from EBV and an ade novirus-transformed cell line that expresses the EBV nuclear antigens- 1 (EBNA-1). As EBV vectors can replicate as episomes in the nuclei, su ch vectors can have a stable transfection efficiency as high as 25% an d provide a straightforward way of obtaining large amounts of recombin ant proteins transiently or stably.