THE ACTIVE TRAPPING OF DOXORUBICIN IN LIPOSOMES BY PH GRADIENT - PHOTON-CORRELATION SPECTROSCOPY AND FLUOROMETRIC STUDY

Authors
MONTERO MT MARTI A HERNANDEZBORRELL J
Citation
Mt. Montero et al., THE ACTIVE TRAPPING OF DOXORUBICIN IN LIPOSOMES BY PH GRADIENT - PHOTON-CORRELATION SPECTROSCOPY AND FLUOROMETRIC STUDY, International journal of pharmaceutics, 96(1-3), 1993, pp. 157-165
Citations number
24
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
International journal of pharmaceutics → ACNP
ISSN journal
03785173
Volume
96
Issue
1-3
Year of publication
1993
Pages
157 - 165
Database
ISI
SICI code
0378-5173(1993)96:1-3<157:TATODI>2.0.ZU;2-P
Abstract
Changes in liposome diameter in response to a pH gradient during uptak e of doxorubicin were studied. After an initial increase liposomes und erwent shrinkage which was dependent on lipid composition and temperat ure of incubation. Changes in fluorescence undergone by the drug durin g the active trapping process were also monitored. The observed self-q uenching of doxorubicin was consistent with the kinetics of drug entra pement within the internal aqueous compartment of the vesicles except when incubation was carried out at 37-degrees-C in the presence of cho lesterol.