DIRECT SEQUENCING OF CONSENSUS PRIMER GENERATED PCR FRAGMENTS OF HUMAN PAPILLOMAVIRUSES

Consensus primers specific for the L1 and E1 regions were used to ampl ify HPV DNA fragments. These fragments were then directly sequenced us ing the consensus primers as the sequencing primers. The linking of PC R amplification, direct sequencing, and computer data bank analysis pr ovides a new approach in the detection of different HPVs and has sever al advantages over existing methodology. These advantages include incr eased precision in the rapid characterization of known HPVs, detection of mutations, and identification of new HPV types.