PRODUCTION OF ECTOMYCORRHIZAL PICEA-ABIES HEBELOMA-CRUSTULINIFORME SEEDLINGS FOR ECOLOGICAL-STUDIES - EFFECTS OF SYNTHESIS TECHNIQUES ON THE MORPHOLOGY OF THE SYMBIOSIS

Various in vitro synthesis techniques were applied to produce numerous ectomycorrhizal Picea abies - Hebeloma crustuliniforme seedlings whic h could be later used for experiments in the field of environmental re search. Included synthesis Methods are the Erlenmeyer flask technique, the cuvette technique, and the growth pouch technique. A mantle and a Hartig net were present in all synthesized and naturally grown symbio ses. Stable features were the white color of the mantle, its two-layer ed structure, and the structure of the Hartig net. A few characters su ch as the thickness of the mantle and the development of the Hartig ne t formation were variable due to the synthesis method. The techniques suitable for producing ectomycorrhizal seedlings are the Erlenmeyer fl ask technique using vermiculite and peat moss and the growth pouch tec hnique: the first technique because of sterility and good ectomycorrhi zal formation within approximately two months. the second technique be cause of direct observation of ectomycorrhizal formation within one to two months and complete removing of ectomycorrhizae including extrama trical mycelia.