3-WAY DIFFERENTIATION OF CHINESE-HAMSTER OVARY CHROMOSOMES BY IMMUNOPEROXIDASE TECHNIQUE USING A MONOCLONAL ANTIBROMODEOXYURIDINE ANTIBODY

Authors
PINERO J ORTIZ T CORTES F
Citation
J. Pinero et al., 3-WAY DIFFERENTIATION OF CHINESE-HAMSTER OVARY CHROMOSOMES BY IMMUNOPEROXIDASE TECHNIQUE USING A MONOCLONAL ANTIBROMODEOXYURIDINE ANTIBODY, Biotechnic & histochemistry, 68(4), 1993, pp. 215-221
Citations number
27
Categorie Soggetti
Cytology & Histology","Biothechnology & Applied Migrobiology
Journal title
Biotechnic & histochemistry → ACNP
ISSN journal
10520295
Volume
68
Issue
4
Year of publication
1993
Pages
215 - 221
Database
ISI
SICI code
1052-0295(1993)68:4<215:3DOCOC>2.0.ZU;2-U
Abstract
A new permanent staining procedure for sister chromatid differentiatio n (SCD) in cultured Chinese hamster ovary cells has been established b y combining the three-way differentiation in third mitosis (M3) chromo somes and the immunoperoxidase reaction developed with 3,3'-diaminoben zidine using a monoclonal anti-bromodeoxyuridine (BrdU) antibody. This procedure allows SCD at very low BrdU concentrations, and the evaluat ion of the sister chromatid exchange frequencies on a per cell-cycle b asis.