EFFECTS OF CO2 INSUFFLATION ON BACTERIAL-GROWTH IN RATS WITH ESCHERICHIA COLI-INDUCED EXPERIMENTAL PERITONITIS

The effects of i.p. CO2 insufflation on bacterial proliferation in a s etting of Escherichia coli-induced experimental peritonitis was studie d in a rat model. Six male Wistar rats were given 0.25 ml of i.p. sali ne and formed the sham operation group. Twenty-four rats were divided into three groups, and all had i.p. E, coli injections. Microorganism counts were taken after 8 h in ail groups. Group I was used as the con trol group. Group 2 (laparoscopy) was insufflated with CO2, and group 3 (laparotomy) had a midline laparotomy. Microorganism counts were rep eated 8 h after the procedures (16 h after i.p. E. coli inoculation). Postoperative microorganism counts were significantly higher in the CO 2 insufflation group (p < 0.05) compared with the control and laparoto my groups and showed an increase, whereas they decreased in the other two groups.