AMYLOSE-ROSE BENGAL COMPLEXES .2. FT-IR AND RAMAN-SPECTROSCOPY STUDY OF THE AMYLOSE-ROSE BENGAL COMPLEX

Fr-IR and Raman spectra of amylose-Rose Bengal in solution and in lyop hilised samples were investigated. In the Rose Bengal molecule, the nu mber and positions of active atoms, i.e., those that interact with amy lose, were determined. The interaction with amylose was found not to a ffect the Rose Bengal quinonoidal structure. Results for the amylose c omplex show considerable hindrance of the rotational vibrations of amy lose functional groups. Moreover, the influence of Rose Bengal on the infrared signals in the structure-sensitive region (950-1200 cm-1) was studied. The FT-IR results indicated the formation of a stiff amylose network induced by Rose Bengal molecules.