GENETIC-ANALYSIS OF THE GENE FOR PORCINE SUBMAXILLARY-GLAND MUCIN - PHYSICAL ASSIGNMENT OF THE MUC AND INTERFERON-GAMMA GENES TO CHROMOSOME-5

Genetic variation at the porcine submaxillary gland mucin (MUC) locus was revealed by RFLP analysis. A three-allele system was defined, and codominant inheritance was confirmed using a three-generation pedigree comprising 10 founder individuals, 26 F1 animals, and 200 F2 offsprin g. Linkage analysis was performed against a set of 56 genetic markers. The mucin locus was assigned to a previously reported linkage group c omprising the markers interferon gamma (IFNG), diacylglycerol kinase ( DAGK), and an anonymous microsatellite (S0092). Furthermore, the genes for mucin and interferon gamma were physically localized to porcine c hromosome 5q2.3 and 5p1.2-q1.1 by fluorescence and radioactive in situ hybridization, respectively, thereby assigning four new markers to ch romosome 5. This chromosome shares homologies with chromosome 5 in cat tle and chromosome 12 in humans.