IMMUNOCYTOCHEMICAL ANALYSIS OF POLY-BETA-HYDROXYBUTYRATE (PHB) SYNTHASE IN ALCALIGENES-EUTROPHUS H16 - LOCALIZATION OF THE SYNTHASE ENZYME AT THE SURFACE OF PHB GRANULES

Authors
GERNGROSS TU REILLY P STUBBE J SINSKEY AJ PEOPLES OP
Citation
Tu. Gerngross et al., IMMUNOCYTOCHEMICAL ANALYSIS OF POLY-BETA-HYDROXYBUTYRATE (PHB) SYNTHASE IN ALCALIGENES-EUTROPHUS H16 - LOCALIZATION OF THE SYNTHASE ENZYME AT THE SURFACE OF PHB GRANULES, Journal of bacteriology, 175(16), 1993, pp. 5289-5293
Citations number
32
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
175
Issue
16
Year of publication
1993
Pages
5289 - 5293
Database
ISI
SICI code
0021-9193(1993)175:16<5289:IAOP(S>2.0.ZU;2-U
Abstract
Antibodies raised against the Alcaligenes eutrophus poly-beta-hydroxyb utyrate (PHB) synthase polypeptide were used for immunocytochemical lo calization of the synthase enzyme in whole cells and purified PHB gran ules. The data presented demonstrate for the first time that the synth ase enzyme is located on the surface of the PHB granule rather than be ing incorporated inside the granule during its formation. From these b asic observations and data from the recent literature, a model of gran ule assembly is proposed.