Axial trapping forces exerted on microspheres are predicted using a Ga
ussian beam electromagnetic field model and a ray-optics model, and co
mpared with experimental measurements. Ray-optics predicts a maximum t
rapping efficiency Q = -0.14 for optically trapped polystyrene microsp
heres in water, compared to a measured value of -0.12 +/- 0.014 for 10
mum diam microspheres. When the microspheres are composed of amorphou
s silica, the predicted ray-optics Q decreases to -0.11, compared to a
Q = -0.034 predicted by the electromagnetic field model, and a measur
ed value of -0.012 +/- 0.001 for 1 mum diam microspheres. These result
s indicate that the two models have applicability in two different siz
e regimes, and thus, are complementary.