A MOLECULAR, ISOZYME AND MORPHOLOGICAL MAP OF THE BARLEY (HORDEUM-VULGARE) GENOME

Authors
KLEINHOFS A KILIAN A MAROOF MAS BIYASHEV RM HAYES P CHEN FQ LAPITAN N FENWICK A BLAKE TK KANAZIN V ANANIEV E DAHLEEN L KUDRNA D BOLLINGER J KNAPP SJ LIU B SORRELLS M HEUN M FRANCKOWIAK JD HOFFMAN D SKADSEN R STEFFENSON BJ
Citation
A. Kleinhofs et al., A MOLECULAR, ISOZYME AND MORPHOLOGICAL MAP OF THE BARLEY (HORDEUM-VULGARE) GENOME, Theoretical and Applied Genetics, 86(6), 1993, pp. 705-712
Citations number
42
Categorie Soggetti
Genetics & Heredity
Journal title
Theoretical and Applied Genetics → ACNP
ISSN journal
00405752
Volume
86
Issue
6
Year of publication
1993
Pages
705 - 712
Database
ISI
SICI code
0040-5752(1993)86:6<705:AMIAMM>2.0.ZU;2-C
Abstract
A map of the barley genome consisting of 295 loci was constructed. The se loci include 152 cDNA restriction fragment length polymorphism (RFL P), 114 genomic DNA RFLP, 14 random amplified polymorphic DNA (RAPD), five isozyme, two morphological, one disease resistance and seven spec ific amplicon polymorphism (SAP) markers. The RFLP-identified loci inc lude 63 that were detected using cloned known function genes as probes . The map covers 1,250 centiMorgans (cM) with a 4.2 cM average distanc e between markers. The genetic lengths of the chromosomes range from 1 24 to 223 cM and are in approximate agreement with their physical leng ths. The centromeres were localized to within a few markers on all of the barley chromosomes except chromosome 5. Telomeric regions were map ped for the short (plus) arms of chromosomes 1, 2 and 3 and the long ( minus) arm of chromosomes 7.