SOLID-PHASE SYNTHESIS OF O-MANNOSYLATED PEPTIDES - 2 STRATEGIES COMPARED

A comparison of the O-glycosylation of resin-bound assembled peptides with the incorporation of glycosylated amino acids using established c hemistry is presented. Fmoc/tert-butyl-based protecting groups were us ed for the peptidic moieties in conjunction with acetyl sugar protecti on. Koenigs-Knorr glycosylations were carried out using protected brom omannose derivatives, the acceptor being threonine or serine, either i n solution or within a resin-bound peptide. The characterisation of mi crogram quantities of glycopeptides by the use of glycosidases in comb ination with mass spectrometry is also described. (C) Munksgaard 1993.