The microbial colonization of 44 Blom-Singer valves obtained from 16 p
atients over an 11-month period was investigated both by standard micr
obial culture methods and scanning electron microscopy (SEM). The valv
es were all removed from the patients after failure to function correc
tly. The microbiology cultures revealed that Candida species and Staph
ylococcus aureus were present either individually or in combination on
43 valves. SEM showed yeast hyphae attached to and penetrating the su
rfaces of the 5 valves examined. Colonies of staphylococci were also s
een on 2 of these 5 valves. Unlike similar investigations on other voi
ce prostheses, S aureus colonization was associated with Candida colon
ization and valve failure.