Forty-eight Angus x Hereford yearling steers were used to assess the e
ffect of time on feed (TOF) on the nutrient composition of beef longis
simus muscle (LM). Steers were fed a high-concentrate diet with the ex
ception of the d-0 group, which served as a grass-fed control, and the
n were serially slaughtered at 28-d intervals during the 196-d feeding
period. Steaks were removed from the 10th rib and trimmed of exterior
fat and epimysial connective tissue before nutrient analysis. Intramu
scular fat content doubled (P < .05) between d 84 and 112 but did not
differ (P > .05) from d 0 to 84 or from d 112 to 196. This increase in
fat content resulted in decreased (P < .05) concentrations of moistur
e, protein, and ash in the LM. Concentrations of Mg, K, and Fe in the
LM increased (P < . 10) with advanced TOF. The increase in the total l
ipid (TL) content of the LM stemmed from a proportional increase (P <
.05) in neutral lipid (NL). Polar lipid (PL) remained constant (P = .3
3) throughout TOF. The NL and TL became more unsaturated as TOF increa
sed, primarily due to a linear (P < .01) increase in oleic (C 18:1) ac
id concentration. In contrast, the polyunsaturated fatty acid (PUFA) c
oncentration in the PL exhibited a linear (P < .01) decrease across TO
F. As a result, advanced TOF increased the monounsaturated fatty acid
(MUFA) content by 22% and decreased the PUFA content by 72% in the LM.
The ratio of hypercholesterolemic (C14 + C16):hypocholesterolemic (MU
FA + PUFA) fatty acids was unaffected by increasing TOF from d 28 to 1
96; however, this ratio was lower (P < .05) for grass-fed controls (d
0) than for d 28 to 84 and d 196. Cholesterol content (milligrams/100
grams) changed cubically (P = .06) across TOF. Ultimately, by limiting
TOF to 112 d, the beef industry could provide consumers a palatable b
eef product that easily fits into a healthy diet and at the same time
diminishes the costs associated with external fat trim.