The bioavailability of threonine in solvent-extracted soybean meal for
10- to 20-kg pigs was determined using the slope-ratio method. In Exp
. 1, the assay range was determined by feeding six diets to 144 pigs.
The basal diet (.40% threonine) contained corn, soybean meal, and corn
gluten meal. Five additional diets were formulated by supplementing t
he basal diet with .05 to .25% crystalline L-threonine in .05% increme
nts. Weight gain, gain/feed, and plasma concentrations of threonine an
d urea responded quadratically (P < .05) to increasing dietary threoni
ne. Breakpoints ranged from .51 to .54% dietary threonine. Experiment
2 consisted of seven trials in which a total of 239 pigs were used in
a randomized complete block design. Pigs were penned individually and
had ad libitum access to feed and water during the 21-d experiment. Th
e same basal diet that was used in Exp. 1 was supplemented with .018,
.035, .053, or .070% threonine from either L-threonine or soybean meal
. The weight gains of the pigs were partitioned to yield the response
due to the supplemental threonine ingested. Multiple regression was pe
rformed on partitioned weight gain vs supplemental threonine intake, a
nd the assay was tested for validity. The regression lines for L-threo
nine and soybean meal were linear (P < .05) and the intercepts were no
t significantly different (P > .10). The slope ratio for soybean meal:
L-threonine was .80. Although the difference between the soybean meal
and L-threonine slopes was not significant (P > .23), the best estimat
e of the bioavailability of threonine in soybean meal relative to that
of L-threonine was 80%.