INHIBITORY FACTORS OF SERUM OPSONIC ACTIVITY IN SERUM FROM PATIENTS WITH CHRONIC-RENAL-FAILURE

We investigated the mechanism of impaired serum opsonic activity in pa tients suffering from chronic renal failure (CRF). Prehemodialysis poo led serum from CRF patients was fractionated using Sephadex G-50 gel c hromatography, and the effects of the obtained fractions on serum opso nic activity were examined using luminal-dependent chemiluminescence r esponses. Number 25-30 fractions in the prehemodialysis serum containe d factors that may inhibit serum opsonic activity. The presumed molecu lar weight of these fractions is between 300 and 2800 daltons. These f indings indicate that the inhibitory factors of serum opsonic activity may belong to middle molecules.