STUDY ON SEPARATION OF CONALBUMIN AND LYSOZYME FROM HIGH-CONCENTRATION FRESH EGG-WHITE AT HIGH-FLOW RATES BY A NOVEL ION-EXCHANGER

In this report, we show that it is possible to separate valuable prote ins from egg-white using a Productiv(TM) CM ion-exchanger column opera ted at flow rates significantly higher than those than can be achieved using traditional particulate adsorbents. In the approach taken, samp le pretreatment is restricted to a simple dilution of the egg-white, w hich can then be applied to the column at superficial velocities (V(s) ) of up to 13.8 m/h. Under a loading of 220 mg total protein per milli liter of ion-exchanger, the resolution (R(s)) between the eluted conal bumin and lysozyme fractions was found to be almost constant during ni ne consecutive adsorption/desorption cycles. For all nine consecutive batches, the column average adsorption capacity was greater than 30 mg /mL, with 90% recovery of adsorbed protein being achieved in each run. The overall productivity achieved was 12.6 kg/m3 h for lysozyme and 3 1.2 kg/m3 h for conalbumin. (C) 1993 John Wiley & Sons, Inc.