Cl. Norris et Ks. Peters, A PHOTOACOUSTIC CALORIMETRY STUDY OF HORSE CARBOXYMYOGLOBIN ON THE 10-NANOSECOND TIME-SCALE, Biophysical journal, 65(4), 1993, pp. 1660-1665
The development of a photoacoustic calorimeter with a time resolution
of 10 ns is presented, and the dynamics of the enthalpy and volume cha
nges found in the photodissociation of CO from horse carboxymyoglobin
are examined. With this enhanced time resolution a new transient speci
es, the lifetime of which is 29 ns at 20-degrees-C, is observed in the
ligand dissociation process.