IN-VITRO BINDING OF PASTEURELLA-HAEMOLYTI CA TO TRACHEAL MUCUS AND TOA TRACHEAL EPITHELIAL-CELL WALL PREPARATION OF CATTLE

Outer membrane protein preparations (OMP) of 11 Pasteurella haemolytic a field isolates of the serovars A1, A2, A11 and non-typable strains f rom cattle were extracted by N-lauryl-sarcosine sodium salt. Capsular extracts were prepared by heat treatment. Both preparations and whole cell suspensions bound to a preparation of an epithel cell wall fracti on of trachea and to tracheal mucus of cattle. Bindung was demonstrate d by enzyme linked immunosorbent assay (ELISA). Distinct high binding values were shown by the OMP and the capsular extracts of the serovar A1-strains.