BIOLOGICALLY-ACTIVE SECONDARY METABOLITES OF BARLEY .3. A METHOD FOR IDENTIFICATION AND QUANTIFICATION OF HORDENINE AND GRAMINE IN BARLEY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A method was devised for the extraction and quantification of hordenin e and gramine from barley (Hordeum vulgare) tissue using HPLC techniqu es. Quantification was by peak area, the relationship between peak are a and concentration of authentic standards being linear for both horde nine and gramine. Significant differences in the ability of three line s of barley to produce hordenine and gramine were detected using this method.