AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR MONITORING RODENT COLONIES FOR STREPTOBACILLUS-MONILIFORMIS ANTIBODIES

Citation
R. Boot et al., AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR MONITORING RODENT COLONIES FOR STREPTOBACILLUS-MONILIFORMIS ANTIBODIES, Laboratory animals, 27(4), 1993, pp. 350-357
Citations number
21
Categorie Soggetti
Veterinary Sciences
Journal title
ISSN journal
00236772
Volume
27
Issue
4
Year of publication
1993
Pages
350 - 357
Database
ISI
SICI code
0023-6772(1993)27:4<350:AE(FMR>2.0.ZU;2-M
Abstract
An enzyme-linked immunosorbent assay (ELISA) to measure Streptobacillu s moniliformis antibodies in mice and rats was developed. Twelve S. mo niliformis strains originating from cases of rat-bite fever and Haverh ill fever in man and from various rodent species, showed considerable serological relationship. The ELISA appeared specific since antibodies to S. moniliformis were absorbed by autologous and homologous antigen , but not by heterologous bacterial antigens. Acholeplasma laidlawii s howed partial serological relationship with S. moniliformis. The ELISA was validated using experimental infections in mice and rats. These s tudies and observations in naturally infected feral rats, confirmed th at S. moniliformis is difficult to grow on primary isolation, and that the ELISA for S. moniliformis antibodies revealed more contaminated a nimals than culture.