REGION OF THE DROSOPHILA-MELANOGASTER GEN E PRUNE IS TRANSCRIPTIONALLY SATURATED

Using Northern analysis three transcription areas are detected in the gene prune region from which three different mRNAs 1.8, 1.75 and 1.0 k b in length transcribe. Corresponded cDNAs are cloned. Only 1.8 kb mRN A is shown to be changed in size and quantity in the case of the pn1 a nd pn3 mutations. This mRNA seems to be a gene prune transcript. From the analysis of the portion of cDNA cl.1 nucleotide sequence correspon ded 1.75 kb mRNA follows that its putative amino acid product contains a heme-binding site which is characterized for P450 cytochrome superf amily. On the basis of presented results and published data by Teng et al [1] it may be concluded that at least five different mRNAs transcr ibe in the gene prune region in area spanning about 10 kb.