MAPPING OF CLONED RECEPTOR GENE FRAGMENTS OF THE MACROPHAGE-COLONY-STIMULATING FACTOR (FMS) FROM NONAMPLIFIED LIBRARY OF HUMAN-LEUKOCYTE GENES

For investigation of FMS gene polymorphism and mutations that reveal f unctionally meaning in leukemia and myelodisplastic disorders the over lapping recombinants lambda-clones inserted by FMS gene fragments have been obtained from human leukocyte genomic library in the EMBL 3A pha ge by using oligonucleotide prode (27 nucleotides) based on 12 exon of the FMS gene. 15 DNA probes were prepared by subcloning the lambda-cl ones obtained in the pBSKS+ plasmid. The probes obtained allow to anal yse extracellular, transmembrane and tyrosine kinase regions of the FM S gene independently.