ANALYSIS OF THE SQUALESTATINS BY ONLINE PACKED CAPILLARY LIQUID-CHROMATOGRAPHY COMBINED WITH ELECTROSPRAY MASS-SPECTROMETRY

Authors
LANE SJ BRINDED KA TAYLOR NL WATKINS PJF HARRISON ME
Citation
Sj. Lane et al., ANALYSIS OF THE SQUALESTATINS BY ONLINE PACKED CAPILLARY LIQUID-CHROMATOGRAPHY COMBINED WITH ELECTROSPRAY MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 7(10), 1993, pp. 953-956
Citations number
5
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
7
Issue
10
Year of publication
1993
Pages
953 - 956
Database
ISI
SICI code
0951-4198(1993)7:10<953:AOTSBO>2.0.ZU;2-C
Abstract
A mixture of four squalestatins was separated and characterized by gra dient packed capillary liquid chromatography combined with electrospra y collision-induced dissociation mass spectrometry (ESI/CID/MS). Negat ive-ion ESI was found to afford spectra typified by strong pseudo-mole cular ions but providing little structural information. Structural inf ormation was obtained using an ion-focusing RF-only octapole lens to p rovide conditions for CID. The new method showed improvements in sensi tivity when compared to existing thermospray and dynamic liquid second ary-ion mass spectrometry methods.