BEADED MATRICES FROM CROSS-LINKED ALGINATE FOR AFFINITY AND ION-EXCHANGE CHROMATOGRAPHY OF PROTEINS

To obtain a low cost, beaded chromatographic matrix, calcium alginate beads were cross-liked with epichlorohydrin, and calcium was removed b y sodium citrate treatment. The cross-linking reaction for obtaining s table beads was optimized. For purification of haemoglobin by ion exch ange, cross-linked calcium-free alginate beads have a q(max) of 60 mg/ ml and a K(d) of 0.02 mg/ml gel, while for affinity polygalacturonase purification K(aff) was 0.007 ml/g.