K. Tsuchiya et al., ISOLATION OF ELECTRIC PULSE-INDUCED ESCHERICHIA-COLI GENES BY OPERON FUSION TECHNIQUES, Biotechnology techniques, 7(10), 1993, pp. 719-722
A search of electric-pulse induced genes in Escherichia coli was carri
ed out by operon fusion techniques with a hybrid bacteriophage Mu, whi
ch creates transcriptional fusions of the structural gene of beta-gala
ctosidase to the host. Among two hundred transductants tested, nine co
lonies showed higher expression of more than twofold when they were tr
eated with electric pulses. An electric-pulse stimulated transductant
was not stimulated by UV irradiation, which is known to induce an SOS
response. Conversely, strain PQ37, which has an operon fusion in one o
f the SOS genes, did not respond to an electric pulse treatment. A pos
sibility of the presence of the electric-pulse stimulated genes, which
were not induced by DNA damages, was suggested.