STUDIES ON PLANT-TISSUE CULTURES .96. CONSTRUCTION OF INTEGRATED SYSTEM FOR SELECTION OF FUSED PLANT-PROTOPLASTS

Authors
SAKAMOTO K FUJITA K HIRAYAE K IIDA K KOYANO T ASADA Y FURUYA T
Citation
K. Sakamoto et al., STUDIES ON PLANT-TISSUE CULTURES .96. CONSTRUCTION OF INTEGRATED SYSTEM FOR SELECTION OF FUSED PLANT-PROTOPLASTS, Biotechnology techniques, 7(10), 1993, pp. 749-754
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
10
Year of publication
1993
Pages
749 - 754
Database
ISI
SICI code
0951-208X(1993)7:10<749:SOPC.C>2.0.ZU;2-4
Abstract
An integrated system has been constructed to instantly identify and ef ficiently sort the heterokaryons formed by plant protoplast fusion. Th e system is composed of the following functions: a) a transport system , b) an electro-manipulator, c) a cell harvester, d) a flow cytometer/ cell sorter, and e) a control device. The conditions for an efficient and reproducible enrichment of the heterokaryons have been investigate d by this system using the fluorescein isothiocyanate stained protopla sts preparing from Glycyrrhiza glabra cell cultures and unstained prot oplasts of Abrus precatorius cell cultures which contain a large quant ity of chlorophyll.