EVALUATION OF MONOCLONAL-ANTIBODIES WITH SPECIFICITY FOR THE HUMAN PLATELET HPA-1 ALLOTYPES

Two mouse monoclonal antibodies have been characterized. Both antibodi es specifically recognized the amino acid sequences which are responsi ble for the presence of the HPA-la and HPA-lb alloantigens at the surf ace of human platelet. The monoclonality allied with an exquisite spec ificity of these antibodies allow a perfect typing of platelet HPA-1 s tatus. A fast and reliable Elisa assay which fulfills all the needs an d requirements of hematology laboratories has been set up and is descr ibed here. The Elisa assay has been tested on a population of 689 bloo d donors. The results agreed with the known phenotypic frequencies of the HPA-1 alloantigens.