SEQUENCES OF TRANSFER RNA-ENCODING GENES AND ASSOCIATED OPEN READING FRAMES OF STREPTOMYCES-LIVIDANS

A gene library of Streptomyces lividans has been screened for tRNA-enc oding genes with labeled Streptomyces tRNA as a probe. By sequence ana lysis of hybridizing fragments, two single genes have been identified which code for tRNA(Asp) and tRNA(Gly). Associated with the tRNA(Gly) gene, there are three open reading frames (ORFs) which might code for gene products possibly involved in active transport processes through the bacterial membrane. The transcriptional organization of tRNA(Gly) and the following ORFs was examined by high-resolution S1 mapping. A t hird clone carried a cluster of genes which encode two tRNA(Gln) and t hree tRNA(Glu). This cluster corresponds to a similar cluster previous ly described for Streptomyces rimosus [Plohl and Gamulin, Mol. Gen. Ge net. 222 (1990) 129-134].