A whole-watershed manipulation project was begun on the Fernow Experim
ental Forest in West Virginia, USA, in 1987, with the objective of inc
reasing understanding of the effects of acidic deposition on forest ec
osystems. Two treatment watersheds (WS9 and WS3) and one control water
shed (WS4) were included. Treatments were twice-ambient N and S deposi
tion, applied via NH4SO4 fertilizer, with three applications per year.
Three years of pretreatment data were collected and used for calibrat
ion. Stream water chemistry data collected during 3 years of treatment
were evaluated. Stream water pH and electrical conductivity were not
significantly affected by the elevated N and S inputs on either treatm
ent watershed. On WS9, there were no statistically significant treatme
nt effects on stream water export of Ca, SO4, or NO3. On WS3, however,
stream export of both NO3 and Ca have increased as a result of acidif
ication treatments. The implications of these results are discussed. R
esearch is continuing so that the processes involved may be elucidated
. In addition, effects on vegetation, aquatic invertebrates and amphib
ians also are being evaluated.