STRUCTURAL CHARACTERIZATION OF FERULOYL OLIGOSACCHARIDES FROM SPINACH-LEAF CELL-WALLS

Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobios e, each carrying a ferulic acid moiety. The oligosaccharides were char acterized by NMR spectroscopy, methylation analysis, and FABMS. They w ere O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1 --> 5)-L-arabin ofuranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl-(1 --> 4)-D-g alactopyranose, and O-alpha-L-arabinofuranosyl-(1 --> O-(2-O-trans-fer uloyl-alpha-L-arabinofuranosyl)-(1 --> 5)-L-arabinofuranose.