STRUCTURAL COMPARISON OF THE O4-SPECIFIC POLYSACCHARIDES FROM ESCHERICHIA-COLI O4-K6 AND ESCHERICHIA-COLI O4-K52

Two distinct forms of the O4 antigen (LPS) from E. coli were analysed by H-1 and C-13 NMR spectroscopy. Both consisted of D-glucose, L-rhamn ose, 2-acetamido-2,6-dideoxy-L-galactose (L-FucNAc), and 2-acetamido-2 -deoxy-D-glucose. Their structures were found to be [GRAPHICS] In the O4-specific polysaccharide from E. coli O4: K3, O4: K6, and O4: K12, X is alpha-D-Glcp. In the O4 specific polysaccharide from E. coli O4: K 52, the rhamnose residue is not substituted (X = H).